Please use this identifier to cite or link to this item: https://scholar.ptuk.edu.ps/handle/123456789/559
Title: Characterization of bacterial populations in Danish raw milk cheeses made with different starter cultures by denaturating gradient gel electrophoresis and pyrosequencing
Authors: Masoud, Wafa
Keywords: DGGE
Pyrosequencing
Issue Date: 2011
Publisher: Elsevier
Series/Report no.: ;142-148
Abstract: The bacterial populations in Danish raw milk cheeses were identified using denaturating gradient gel electrophoresis (DGGE) of PCR amplicons of the V3 region of the 16S rRNA gene and pyrosequencing of tagged amplicons of the V3 and V4 regions of the 16S rRNA gene. Both DNA and RNA extracted from cheeses were studied in order to determine the metabolically active bacteria. The main bacteria, which included Lactococcus, Lactobacillus and Streptococcus, were detected by pyrosequencing and DGGE in both 16S rDNA and cDNA obtained from cheeses indicating their viability and contribution to cheese ripening. Other bacteria like Corynebacterium, Halomonas, Pediococcus, Micrococcus and Staphylococcus, which were encountered in some cheese samples at low percentages compared with the total bacterial populations, were only detected by pyrosequencing. 16S rRNA gene pyrosequencing is an efficient method for deep sequencing of microbial communities and it expands our knowledge of the bacterial diversity in raw milk cheese.
URI: https://scholar.ptuk.edu.ps/handle/123456789/559
Appears in Collections:Literature Faculty

Files in This Item:
File Description SizeFormat 
Pyrosequencing and DGGE.pdf471.45 kBAdobe PDFThumbnail
View/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.